Journal: Science Advances

Article Title: Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss

doi: 10.1126/sciadv.aax1387

Figure Lengend Snippet: ( A to L ) Administration of BAY 60-6583 and vehicle for 8 weeks in OVX animals (4 weeks after ovariectomy). Groups are compared to healthy control with no surgery and no treatment (CTL). (A) TRAP staining (purple). Scale bar, 50 μm. (B) Quantification of TRAP-positive cells on bone surface. n = 4. (C) Double-fluorescence bone labeling by calcein (green) and alizarin complexone (red). Scale bar, 100 μm. (D) Quantification of mineral apposition rate (MAR) from bone labeling images. (E) Quantification of bone formation rate (BFR/BS) from bone labeling images. n = 5. (F) Reconstructed microCT images of L4 vertebra. Scale bar, 500 μm. (G to J) Quantification of microCT images. (G) BMD. (H) BV/TV. (I) Tb.N. (J) Tb.Sp. n = 5. Mechanical measurement for (K) maximum load and (L) stiffness of tibia. n = 12. OV, ovariectomy, vehicle [dimethyl sulfoxide (DMSO)]; OB, ovariectomy, BAY 60-6583 (1 mg/kg). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The reconstruction of scanned images was performed using microCT Evaluation Program V6.6 (Scanco Medical), followed by generation of radiographs and three-dimensional models using microCT Ray V4.0 (Scanco Medical).

Techniques: Control, Staining, Fluorescence, Labeling

Journal: Medical Physics

Article Title: TLD assessment of mouse dosimetry during microCT imaging

doi: 10.1118/1.2959847

Figure Lengend Snippet: MicroCT PMMA anesthesia support module. Radiation exposures were defined for microCT scans when conducted with and without the use of the microCT PMMA anesthesia support module. The anesthesia module provides gas anesthesia under a HEPA filtered pathogen-free environment.

Article Snippet: The authors wish to thank Dr. Michael J. Paulus from Siemens Pre-Clinical Solutions for providing information regarding the microCT unit and the x-ray source block, Mr. Richard Poelling from the Radiation Safety Office at the Harry S. Truman Memorial VA Hospital, Columbia, MO for providing the ion chambers employed in this investigation.

Techniques:

Journal: Medical Physics

Article Title: TLD assessment of mouse dosimetry during microCT imaging

doi: 10.1118/1.2959847

Figure Lengend Snippet: Internal adsorbed doses of different mouse organs. Table provides the dose measured following a single microCT scan by TLD chips implanted in various organs in a mouse. Radiation dose was measured employing a typical microCT scan protocol. The average absorbed dose over all measurements to the organs is 76±5.0 mGy ( n =3).

Article Snippet: The authors wish to thank Dr. Michael J. Paulus from Siemens Pre-Clinical Solutions for providing information regarding the microCT unit and the x-ray source block, Mr. Richard Poelling from the Radiation Safety Office at the Harry S. Truman Memorial VA Hospital, Columbia, MO for providing the ion chambers employed in this investigation.

Techniques:

Journal: Medical Physics

Article Title: TLD assessment of mouse dosimetry during microCT imaging

doi: 10.1118/1.2959847

Figure Lengend Snippet: MicroCT images of implanted TLDs. MicroCT reconstructed images highlighting the precise locations of implanted TLDs. Figure shows (A) a sagittal microCT slice, (B) a coronal microCT slice, and (C) the axial microCT slice corresponding to the intersected lines in (A) and (B).

Article Snippet: The authors wish to thank Dr. Michael J. Paulus from Siemens Pre-Clinical Solutions for providing information regarding the microCT unit and the x-ray source block, Mr. Richard Poelling from the Radiation Safety Office at the Harry S. Truman Memorial VA Hospital, Columbia, MO for providing the ion chambers employed in this investigation.

Techniques: